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Expression and developmental regulation of the k-FGF oncogene in human and murine embryonal carcinoma cells

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Expression and developmental regulation of the k-FGF oncogene in human and murine embryonal carcinoma cells

Auteurs : Jay Tiesman [États-Unis] ; Angie Rizzino [États-Unis]

Source :

In Vitro Cellular & Developmental Biology [ 0883-8364 ] ; 1989-12-01.

RBID : ISTEX:543D8B8D5E16BD8802107ACB6BCEF5AE9F4E934A

English descriptors

Abstract

Summary: Embryonal carcinoma (EC) cells provide an effective model system for studying growth factor production and regulation during mammalian embryogenesis. Our earlier data indicated that the mouse EC cell lines F9 and PC-13 and the human EC cell line NT2/D1 produce a factor with properties similar to those ascribed to members of the fibroblast growth factor (FGF) family and that production of this FGF-related factor is suppressed when all three EC cell lines are induced to differentiate. Subsequent studies suggested that NT2/D1 EC cells express transcripts for basic FGF (bFGF). The current study confirms and extends these findings using a combination of reverse transcription and polymerase chain reaction (RT-PCR). In this study, the expression of bFGF and other members of the FGF family have been examined in F9 and PC-13 cells in addition to NT2/D1 EC cells. In contrast to NT2/D1 EC cells, bFGF expression could not be detected in F9 and PC-13 EC cells. Additionally, expression of four other members of the FGF family (acidic FGF, int-2, FGF-5, and FGF-6) were not detected in NT2/D1, F9, or PC-13 EC cells. However, expression of another member of the FGF family, the k-FGF oncogene, was detected in NT2/D1, F9, and PC-13 EC cells. Moreover, the expression of this transcript is reduced dramatically when each of the three EC cell lines is induced to differentiate. Taken together, our findings argue that expression of the k-FGF oncogene is predominantly responsible for the FGF-related activity detected in EC cells and that differentiation of these EC cells results in suppression of this oncogene.

Url:

https://api.istex.fr/ark:/67375/1BB-X61XWHLW-0/fulltext.pdf

DOI: 10.1007/BF02621274

Affiliations:

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<keywords scheme="Teeft" xml:lang="en"><term>Bethesda research laboratories</term>
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<term>Bovine</term>
<term>Carcinoma</term>
<term>Cdna</term>
<term>Cell line</term>
<term>Cell lines</term>
<term>Claudio basilico</term>
<term>Current study</term>
<term>Delli bovi</term>
<term>Developmental regulation</term>
<term>Early development</term>
<term>Early mouse embryos</term>
<term>Embryo</term>
<term>Embryonal</term>
<term>Embryonal carcinoma cells</term>
<term>Endonuclease</term>
<term>Ethidium bromide</term>
<term>Fibroblast</term>
<term>Fibroblast growth factor</term>
<term>Fragment</term>
<term>Further evidence</term>
<term>Growth factor</term>
<term>Growth factor activity</term>
<term>Growth factors</term>
<term>Mammalian embryogenesis</term>
<term>Mesoderm formation</term>
<term>Northern blot analysis</term>
<term>Nucleotide sequence</term>
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<term>Oligonucleotide primers</term>
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<term>Subsequent studies</term>
<term>Thermal cycling</term>
<term>Tiesman</term>
<term>Transcript</term>
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<term>York university school</term>
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<front><div type="abstract" xml:lang="en">Summary: Embryonal carcinoma (EC) cells provide an effective model system for studying growth factor production and regulation during mammalian embryogenesis. Our earlier data indicated that the mouse EC cell lines F9 and PC-13 and the human EC cell line NT2/D1 produce a factor with properties similar to those ascribed to members of the fibroblast growth factor (FGF) family and that production of this FGF-related factor is suppressed when all three EC cell lines are induced to differentiate. Subsequent studies suggested that NT2/D1 EC cells express transcripts for basic FGF (bFGF). The current study confirms and extends these findings using a combination of reverse transcription and polymerase chain reaction (RT-PCR). In this study, the expression of bFGF and other members of the FGF family have been examined in F9 and PC-13 cells in addition to NT2/D1 EC cells. In contrast to NT2/D1 EC cells, bFGF expression could not be detected in F9 and PC-13 EC cells. Additionally, expression of four other members of the FGF family (acidic FGF, int-2, FGF-5, and FGF-6) were not detected in NT2/D1, F9, or PC-13 EC cells. However, expression of another member of the FGF family, the k-FGF oncogene, was detected in NT2/D1, F9, and PC-13 EC cells. Moreover, the expression of this transcript is reduced dramatically when each of the three EC cell lines is induced to differentiate. Taken together, our findings argue that expression of the k-FGF oncogene is predominantly responsible for the FGF-related activity detected in EC cells and that differentiation of these EC cells results in suppression of this oncogene.</div>
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Expression and developmental regulation of the k-FGF oncogene in human and murine embryonal carcinoma cells

Expression and developmental regulation of the k-FGF oncogene in human and murine embryonal carcinoma cells

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri